Measuring the structural stiffness goals to disclose the impression of nanostructured parts or varied physiological circumstances on the elastic response of fabric to an exterior indentation. With a pyramidal tip at a nano-scale, we employed the atomic drive microscopy (AFM) to indent the surfaces of two compositions of polyacrylamide gels with totally different softness and seedling roots of Arabidopsis thaliana. We discovered that the stiffness-depth curve derived from the measured drive displays a heterogeneous character in elasticity. In accordance with the tendency of stiffness-depth curve, we decomposed the responding drive into depth-impact (FC), Hookean (FH) and tip-shape (FS) parts, referred to as trimechanic, the place FS and its gradient must be offset on the floor or subsurfaces of the indented materials. Thereby, trimechnic principle permits us to watch how the three restoring nanomechanics change with diverse depth. Their strengths are represented by the respective spring constants (kC, kH, kS) of three parallel-connected spring (3PCS) analogs to distinguish restoring nanomechansims of indented supplies. The efficient Younger’s modulus Ê and the whole stiffness kT (= kH + kS) globally unambiguously distinguish the softness between the 2 gel classes. Knowledge fluctuations had been noticed within the elasticity parameters of particular person samples, reflecting nanostructural variations within the gel matrix. Comparable tendencies had been discovered within the outcomes from rising plant roots, although the info fluctuations are expectedly rather more dramatic. The zone-wise illustration of stiffness by the trimechanic-3PCS framework demonstrates a stiffness measure that displays beneath nanostructures encountered by deepened depth. The trimechanic-3PCS framework can apply any mechanical mannequin of power-law primarily based force-depth relationship and is appropriate with skinny layer corrections. It offers a brand new paradigm for analyzing restoring nanomechanics of soppy biomaterials in response to indenting forces.