Issue 18, 2011
From the journal:

Analyst

Nucleic acid-modulated silver nanoparticles: A new electrochemical platform for sensing chloride ion

Jianyu Jin,a   Xiangyuan Ouyang,b   Jishan Li,b   Jianhui Jiang,b   Hao Wang,a   Yongxiang Wanga  and  Ronghua Yang*ab  

* Corresponding authors

a Beijing National Laboratory for Molecular Sciences, College of Chemistry and Molecular Engineering, Peking University, Beijing, China
E-mail: Yangrh@pku.edu.cn
Fax: +86 731-8882 2523

b State Key Lab of Chemo/Biosensing and Chemometrics, Biomedical Engineering Center, College of Chemistry and Chemical Engineering, Hunan University, Changsha, China

Abstract

Inorganic nanomaterials have generated considerable interest in connection to the design of biosensors. Here we exploit the DNA-induced generation of silver nanoparticles for developing an electrical biosensing protocol for chloride ions. Conjugated with thiol modified oligonucleotide, silver nanoparticles were template-synthesized and immobilized on gold electrode. During cyclic voltammogram (CV) scans, the silver nanoparticles were oxidized at high potential to form a layer of Ag/AgCl complex in the presence of Cl−, giving off sharp solid state redox signals. Under the optimum condition, the electrode responded to Cl− over a dynamic range of 2.0 × 10−5–0.01 M, with a detection limit of 5.0 × 10−6 M. Moreover, the specific solubility product constant-based anion recognition made the electrode applicable at a wide pH range and in complex biological systems. To demonstrate the analytical applications of this sensor in real samples, the Cl− concentrations in human urine were measured without any sample pretreatment. Urinary Cl− detected by the proposed sensor ranged from 110 to 200 mM, which was comparable to the results obtained by standard silver titration.

Graphical abstract: Nucleic acid-modulated silver nanoparticles: A new electrochemical platform for sensing chloride ion

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Article information

DOI
https://doi.org/10.1039/C1AN15283A
Article type
Paper
Submitted
07 Apr 2011
Accepted
05 Jun 2011
First published
26 Jul 2011

Analyst, 2011,136, 3629-3634

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Nucleic acid-modulated silver nanoparticles: A new electrochemical platform for sensing chloride ion

J. Jin, X. Ouyang, J. Li, J. Jiang, H. Wang, Y. Wang and R. Yang, Analyst, 2011, 136, 3629 DOI: 10.1039/C1AN15283A

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