Emerging landscape of cell-penetrating peptide-mediated nucleic acid delivery and their utility in imaging, gene-editing, and RNA-sequencing

Jingping Geng; Xuan Xia; Lin Teng; Lidan Wang; Linlin Chen; Xiangli Guo; Bonn Belingon; Jason Li; Xuemei Feng; Xianghui Li; Wendou Shang; Yingying Wan; Hu Wang

doi:10.1016/j.jconrel.2021.11.032

Emerging landscape of cell-penetrating peptide-mediated nucleic acid delivery and their utility in imaging, gene-editing, and RNA-sequencing

J Control Release. 2022 Jan:341:166-183. doi: 10.1016/j.jconrel.2021.11.032. Epub 2021 Nov 22.

Authors

Jingping Geng¹, Xuan Xia², Lin Teng³, Lidan Wang¹, Linlin Chen⁴, Xiangli Guo¹, Bonn Belingon⁵, Jason Li⁶, Xuemei Feng¹, Xianghui Li¹, Wendou Shang¹, Yingying Wan¹, Hu Wang⁷

Affiliations

¹ Department of Microbiology and Immunology, Medical School, China Three Gorges University, Yichang 443002, China; Hubei Key Laboratory of Tumor Microenvironment and Immunotherapy, China Three Gorges University, Yichang 443002, China.
² Department of Physiology and Pathophysiology, Medical School, China Three Gorges University, Yichang 443002, China.
³ Department of Cardiovascular Medicine, The First Clinical Medical College of China Three Gorges University, Yichang 443002, China.
⁴ Department of Microbiology and Immunology, Medical School, China Three Gorges University, Yichang 443002, China; Hubei Key Laboratory of Tumor Microenvironment and Immunotherapy, China Three Gorges University, Yichang 443002, China; Affiliated Ren He Hospital of China Three Gorges University, Yichang 443002, China.
⁵ Institute of Cell Engineering, Johns Hopkins University, Baltimore, MD 21210, USA.
⁶ Department of Biology, Johns Hopkins University, Baltimore, MD 21210, USA.
⁷ Department of Microbiology and Immunology, Medical School, China Three Gorges University, Yichang 443002, China. Electronic address: biomed_wang@yahoo.com.

PMID: 34822907
DOI: 10.1016/j.jconrel.2021.11.032

Abstract

The safety issues like immunogenicity and unacceptable cancer risk of viral vectors for DNA/mRNA vaccine delivery necessitate the development of non-viral vectors with no toxicity. Among the non-viral strategies, cell-penetrating peptides (CPPs) have been a topic of interest recently because of their ability to cross plasma membranes and facilitate nucleic acids delivery both in vivo and in vitro. In addition to the application in the field of gene vaccine and gene therapy, CPPs based nucleic acids delivery have been proved by its potential application like gene editing, RNA-sequencing, and imaging. Here, we focus on summarizing the recent applications and progress of CPPs-mediated nucleic acids delivery and discuss the current problems and solutions in this field.

Keywords: CPP; Delivery; Gene editing; Gene therapy; Imaging; RNA-sequencing.

Publication types

Research Support, Non-U.S. Gov't
Review

MeSH terms

Cell-Penetrating Peptides*
Gene Editing
Nucleic Acids*
RNA, Small Interfering
Vaccines, Synthetic
mRNA Vaccines

Substances

Cell-Penetrating Peptides
Nucleic Acids
RNA, Small Interfering
Vaccines, Synthetic
mRNA Vaccines