Identification of Cellular MicroRNA Coupling Strand Displacement Polymerization and Nicking‐Endonuclease‐Based Cleavage
P Miao, Y Tang, Q Zhang, B Bo, J Wang - ChemPlusChem, 2015 - Wiley Online Library
P Miao, Y Tang, Q Zhang, B Bo, J Wang
ChemPlusChem, 2015•Wiley Online LibraryA target induced recycling amplification method for the detection of cellular microRNA is
reported based on strand displacement polymerization and nicking endonuclease mediated
cleavage. Moreover, by utilizing silver nanoparticle‐based solid‐state Ag/AgCl reaction,
ultrahigh sensitivity is achieved with powerful discriminating towards the identification of
target microRNA. The limit of detection is as low as 70 am. This method also allows the
detection of cellular microRNA by lysing cells, thus making it a powerful tool for microRNA …
reported based on strand displacement polymerization and nicking endonuclease mediated
cleavage. Moreover, by utilizing silver nanoparticle‐based solid‐state Ag/AgCl reaction,
ultrahigh sensitivity is achieved with powerful discriminating towards the identification of
target microRNA. The limit of detection is as low as 70 am. This method also allows the
detection of cellular microRNA by lysing cells, thus making it a powerful tool for microRNA …
Abstract
A target induced recycling amplification method for the detection of cellular microRNA is reported based on strand displacement polymerization and nicking endonuclease mediated cleavage. Moreover, by utilizing silver nanoparticle‐based solid‐state Ag/AgCl reaction, ultrahigh sensitivity is achieved with powerful discriminating towards the identification of target microRNA. The limit of detection is as low as 70 am. This method also allows the detection of cellular microRNA by lysing cells, thus making it a powerful tool for microRNA expression profiling and disease diagnosis.
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