A region of basic amino-acid cluster in HIV-1 Tat protein is essential forTrans-acting activity and nucleolar localization
SI Endo, S Kubota, H Siomi, A Adachi, S Oroszlan… - Virus genes, 1989 - Springer
The trans-acting factor of human immunodeficiency virus (HIV), Tat, has a basic amino-acid
cluster that is highly conserved among different HIV isolates. We have examined the effects
of mutations in the basic region of Tat on its trans-acting activity and cellular localization.
Introduction of a stop codon immediately preceding the basic region abolished the activity,
while the truncated mutant with the basic region retained some activity. The basic region of
Tat was replaceable with that of Rev (another trans-acting factor of HIV) but not with that of …
cluster that is highly conserved among different HIV isolates. We have examined the effects
of mutations in the basic region of Tat on its trans-acting activity and cellular localization.
Introduction of a stop codon immediately preceding the basic region abolished the activity,
while the truncated mutant with the basic region retained some activity. The basic region of
Tat was replaceable with that of Rev (another trans-acting factor of HIV) but not with that of …
Abstract
Thetrans-acting factor of human immunodeficiency virus (HIV), Tat, has a basic amino-acid cluster that is highly conserved among different HIV isolates. We have examined the effects of mutations in the basic region of Tat on itstrans-acting activity and cellular localization. Introduction of a stop codon immediately preceding the basic region abolished the activity, while the truncated mutant with the basic region retained some activity. The basic region of Tat was replaceable with that of Rev (anothertrans-acting factor of HIV) but not with that of adenovirus Ela nor cellular enzyme. The result of immunofluorescence analysis revealed a correlation between the nuclear, especially nucleolar, accumulation and the activities of mutant Tat proteins.
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